[vc_row][vc_column width=”1\/4″]
During\u00a0stage I<\/strong>\u00a0entitled: \u201eSynthesis and characterization of the precursors for the further<\/strong> The obtained materials were characterized from several points of view for the correct correlation During\u00a0stage II<\/strong>\u00a0entitled: \u201eDesign and synthesis of peptides-functionalized nano \/<\/strong> During\u00a0stage III<\/strong>\u00a0entitled: \u201eCharacterization of nano \/ microparticles functionalized with<\/strong> During\u00a0stage IV<\/strong>\u00a0entitled: \u201eEvaluation of the biomaterial properties of the 2PEP-N\/MPs\u201d several In stage V entitled: "In vitro evaluation of 2PEP-N\/MPs" several activities were carried out as In stage VI<\/strong> entitled: \u201cIn vitro evaluation of apoptosis and anti-inflammatory potential of<\/strong> [vc_row][vc_column width=”1\/4″][\/vc_column][vc_column width=”3\/4″][vc_column_text] Project results During\u00a0stage I\u00a0entitled: \u201eSynthesis and characterization of the precursors for the further preparation of N \/ MPs\u201d several activities were carried out as follows: Act 1.1\u00a0\u2013 Synthesis and characterization of carboxymethyl chitosan (CMCS) Act 1.2\u00a0\u2013 Conjugation of CMCS with CGSPGWVRC and indolicidin peptides Act 1.3\u00a0\u2013 Synthesis and characterization of fluorescein-labeled CMCS […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":5920,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"inline_featured_image":false,"footnotes":""},"class_list":["post-5935","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.univapollonia.ro\/en\/wp-json\/wp\/v2\/pages\/5935","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.univapollonia.ro\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.univapollonia.ro\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.univapollonia.ro\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.univapollonia.ro\/en\/wp-json\/wp\/v2\/comments?post=5935"}],"version-history":[{"count":5,"href":"https:\/\/www.univapollonia.ro\/en\/wp-json\/wp\/v2\/pages\/5935\/revisions"}],"predecessor-version":[{"id":8394,"href":"https:\/\/www.univapollonia.ro\/en\/wp-json\/wp\/v2\/pages\/5935\/revisions\/8394"}],"up":[{"embeddable":true,"href":"https:\/\/www.univapollonia.ro\/en\/wp-json\/wp\/v2\/pages\/5920"}],"wp:attachment":[{"href":"https:\/\/www.univapollonia.ro\/en\/wp-json\/wp\/v2\/media?parent=5935"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\npreparation of N \/ MPs<\/strong>\u201d several activities were carried out as follows:
\nAct 1.1<\/strong>\u00a0\u2013 Synthesis and characterization of carboxymethyl chitosan (CMCS)
\nAct 1.2<\/strong>\u00a0\u2013 Conjugation of CMCS with CGSPGWVRC and indolicidin peptides
\nAct 1.3<\/strong>\u00a0\u2013 Synthesis and characterization of fluorescein-labeled CMCS<\/p>\n
\nof the properties with the synthesis parameters.<\/p>\n
\nmicroparticles (2PEP-N \/ MPs)<\/strong>\u201d several activities were carried out as follows:
\nAct 2.1<\/strong>\u00a0\u2013 Preparation of nano\/microspheres functionalized with peptides
\nAct 2.2<\/strong>\u00a0\u2013 Preparation of nano\/microcapsules functionalized with peptides
\nTwo types of peptide-functionalized particles, nano\/microsphere and nano\/microcapsules, were
\nprepared.<\/p>\n
\npeptides (2PEP-N \/ MPs)<\/strong>\u201d several activities were carried out as follows:
\nAct 3.1<\/strong>\u00a0\u2013 Physico-chemical characterization and correlation of the structure with the properties of
\n2PEP-N \/ MPs
\nAct 3.2<\/strong>\u00a0\u2013 Dexamethasone loading and in vitro release studies<\/p>\n
\nactivities were carried out as follows:
\nAct 4.1<\/strong>\u00a0Enzymatic biodegradability studies
\nAct 4.2<\/strong>\u00a0In vitro evaluation of 2PEP-N\/MPs interaction with blood components
\nAct 4.3<\/strong>\u00a0Protein adsorption studies
\nAct 4.4<\/strong>\u00a0Bioadhesion tests
\nAct 4.5<\/strong>\u00a0Optimization of the preparation process of 2PEP-N\/MPs<\/p>\n<\/div>\n
\nfollows:
\nAct 5.1<\/strong> – Antimicrobial activity of 2PEP-N\/MPs
\nAct 5.2<\/strong> – Synthesis and characterization of fluorescein-labeled 2PEP-N\/MPs
\nAct 5.3<\/strong> – In vitro evaluation of the cytotoxic impact of 2PEP-N\/MPs on both normal lung cells
\nand RAW 264.7 macrophages
\nAct 5.4<\/strong> – Clonogenic assay of the efficacy of 2PEP-N\/MPs therapy on the viability and
\nproliferation of tested cells
\nAct 5.5<\/strong> – Estimation of in vitro cellular uptake of 2PEP-N\/MPs<\/p>\n
\n2PEP-N\/MPs and preliminary in vivo tests\u201d<\/strong> several activities were carried out as follows:
\nAct 6.1<\/strong> – Investigation of the cell apoptosis process in both normal lung cells and RAW 264.7
\nmacrophage cells under the action of 2PEP-N\/MPs
\nAct 6.2<\/strong> – Evaluation of the anti-inflammatory potential of 2PEP-N\/MPs in model cell cultures
\nAct 6.3<\/strong> – Selection of optimal 2PEP-N\/MPs for in vivo tests
\nAct 6.4<\/strong> – In vivo biodistribution assays of fluorescein-labeled 2PEP-N\/MPs[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n","protected":false},"excerpt":{"rendered":"