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Project results<\/strong><\/p>\n Within the first stage entitled: Design, preparation and characterization of curcumin-loaded nanofibers\u201d a Act 3.2 – In vitro release and permeation studies of curcumin from curcumin-loaded nanofibers (NFC) Within the four stage entitled: \u201cEvaluation of the biomaterial properties of the curcumin-loaded Within these activities were carried out: <\/p>\n Within the five stage entitled<\/strong>: \u201cAntimicrobial activity of Act 5.1 – Antimicrobial activity [\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n","protected":false},"excerpt":{"rendered":" [vc_row][vc_column width=”1\/4″][\/vc_column][vc_column width=”3\/4″][vc_column_text] Project results Within the first stage entitled: Design, preparation and characterization of curcumin-loaded nanofibers\u201d a main activity was carried out with the title: Act 1.1 – Preparation and characterization of curcumin-loaded nanofibers (NFC) The obtained nanofibers were characterized structurally (FTIR and XRD analyses), morphologically (SEM) and of their behavior in solutions that […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":6115,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"inline_featured_image":false,"footnotes":""},"class_list":["post-6121","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.univapollonia.ro\/en\/wp-json\/wp\/v2\/pages\/6121","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.univapollonia.ro\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.univapollonia.ro\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.univapollonia.ro\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.univapollonia.ro\/en\/wp-json\/wp\/v2\/comments?post=6121"}],"version-history":[{"count":7,"href":"https:\/\/www.univapollonia.ro\/en\/wp-json\/wp\/v2\/pages\/6121\/revisions"}],"predecessor-version":[{"id":8837,"href":"https:\/\/www.univapollonia.ro\/en\/wp-json\/wp\/v2\/pages\/6121\/revisions\/8837"}],"up":[{"embeddable":true,"href":"https:\/\/www.univapollonia.ro\/en\/wp-json\/wp\/v2\/pages\/6115"}],"wp:attachment":[{"href":"https:\/\/www.univapollonia.ro\/en\/wp-json\/wp\/v2\/media?parent=6121"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\nmain activity was carried out with the title:
\nAct 1.1 – Preparation and characterization of curcumin-loaded nanofibers (NFC) The obtained nanofibers
\nwere characterized structurally (FTIR and XRD analyses), morphologically (SEM) and of their behavior
\nin solutions that mimic biological fluids (swelling degree).
\nWithin the second stage entitled: \u201cDesign, preparation and characterization of biocomposite hydrogel
\ncontaining ZnO NPs (HZ)\u201d a main activity was carried out with the title:
\nAct 2.1 – Preparation and characterization of biocomposite hydrogel containing ZnO NPs (HZ)
\nBiocomposite hydrogels (HZ) obtained within this activity were structurally characterized by FTIR and
\nits surface\/section aspects was determined by SEM. The swelling degree in solutions mimicking
\nbiological fluids (PBS, pH 7.4 and pH 5.4), was determined by gravimetric method.
\nWithin the third stage entitled: \u201cPreparation and characterization of biocomposite hydrogel containing
\nZnO NPs and curcumin-loaded nanofibers (HZ-NFC) and in vitro release and permeation studies of
\ncurcumin \u201d two main activities were carried out with the title:
\nAct 3.1 – Preparation and characterization of biocomposite hydrogel containing ZnO NPs and curcumin-
\nloaded nanofibers (HZ-NFC)<\/p>\n
\nand biocomposite hydrogel type HZ-NFC
\nThe obtained materials within this activity were structurally characterized by FTIR and its morphological
\ncharacteristics were determined by SEM. The swelling degree in solutions mimicking biological fluids
\n(PBS, pH 7.4 and pH 5.4), was determined by gravimetric method.
\nIn vitro release and permeation studies of curcumin from curcumin-loaded nanofibers (NFC) and
\nbiocomposite hydrogel type HZ-NFC were performed on static Franz diffusion cells (Copley
\nvertical diffusion cells system) using cellulose artificial membranes and Strat-M Membrane as
\nmodel membranes. The concentrations of Curcumin in the receiver medium will be determined by
\nUV\u2013Vis spectroscopy (Nanodrop One UV\u2013Vis Spectrophotometer).<\/p>\n
\nnanofibers (NFC), biocomposite hydrogel type HZ and biocomposite hydrogel type HZ-NFC\u201d four main
\nactivities were carried out with the title:
\nAct 4.1 – Biodegradability studies
\nAct 4.2 – In vitro assessment of the interaction of NFC, HZ and of HZ-NFC with the blood components
\nAct 4.3 – Assessment of the in vitro cytotoxicity on human dermal fibroblasts
\nAct 4.4 – The skin irritation potential.<\/p>\n
\n\u2022 Protocol for the biocomposite hydrogel type HZ-NFC preparation with the best biomaterial
\nproperties
\n\u2022 the biodegradation studies of the starting components (nanofibers \u2013 NFC), simple hydrogels with
\nZnO nanoparticles (HZ) and hydrogels containing ZnO nanoparticles and nanofibers (HZ-NFC)
\nin PBS solution (pH = 7.4 and pH 5.4) at temperatures of 37 \u00b1 1\u00b0 C and 40 \u00b1 1\u00b0C;
\n\u2022 In vitro evaluation of the effects of NFC nanofibers, HZ hydrogels and HZ-NFC type hydrogels
\non blood. In this regard, the hemolysis and hemocompatibility test were performed, which
\nincludes the prothrombin time (PT), Quick Index (IQ) and International Normalized Ratio (INR).
\n\u2022 Evaluation of the toxicity degree of nanofibers (NFC), hydrogels containing ZnO nanoparticles
\n(HZ) and biocomposite hydrogels containing both ZnO nanoparticles and nanofibers (HZ-NFC)
\non the adult human dermal fibroblast cell line (HDFa)
\n\u2022 The irritant potential induced by NFC, HZ and HZ-NFC formulations using reconstructed human
\nepidermis – is being evaluated and will be delivered by the end of stage 4 (31.12.2023)<\/p>\n
\nNFC, HZ and biocomposite hydrogel type HZ- NFC and optimization and selection of biocomposite hydrogels<\/em>\u201d four main activities were carried out
\nwith the title:<\/p>\n
\nAct 5.2 – Optimization of the experimental conditions and selection of biocomposite hydrogels<\/p>\n<\/div>\n